PF4 anticorps (Biotin)

N° du produit ABIN954176

L’anticorps Souris Monoclonal anti-PF4 a été validé pour WB et EIA. Il convient pour détecter PF4 dans des échantillons de Humain.

Aperçu rapide pour PF4 anticorps (Biotin) (ABIN954176)

Antigène: PF4 (Platelet Factor 4 (PF4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PF4 est conjugé à/à la Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: KKO

Détail du produit anti-PF4 anticorps (Biotin)

Specificité: This antibody is specific for Human Platelet Factor IV/heparin complexes. AM31851BT-N (clone KKO), unlike AM31850BT-N (clone RTO) which binds PF4 alone, is specific for PF4/heparin complexes and is seen to have similar binding properties as auto-antibodies found in HIT/HITT.

Réactivité croisée (Details): Species reactivity (tested):Human.

Immunogène: Human Factor IV purified from the supernatant of Thrombin activated platelets.

Isotype: IgG2b

Information d'application

Indications d'application: ELISA: Use at 0.08 μg/mL following coating plate with PF4/ heparin complex. This can be done by incubating PF4 with an excess of heparin (100-200 U/mL), while coating the plate.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur PF4

Antigène: PF4 (Platelet Factor 4 (PF4))

Autre désignation: Platelet Factor 4 / PF4

Sujet: Platelet Factor IV (PF4) is a 70 aa protein released from the alpha granules of activated platelets. PF4 is synthesized by bone marrow megakaryocytes and stored in alpha granules as a non-covalent bound tetramer. Platelet factor IV binds with high affinity to heparin and plays a role in inflammation and wound repair. PF4 is a chemoattractant for neutrophils, monocytes and fibroblasts and has been reported to be an immunologic regulator that inhibits suppressor T-cell activity. Heparin-Induced Thrombocytopenia/Thrombosis (HIT/HITT) is a life-threatening complication that manifests itself in a small population of patients exposed to intravenous-heparin. It is characterized by the production of PF4/heparin auto-antibodies. These auto-antibodies bind the PF4/heparin complexes and subsequently bind the FCGIIa receptor on the platelets surface through their Fc region. This activates the platelets and can initiate clot formation.Synonyms: C-X-C motif chemokine 4, CXCL4, Iroplact, Oncostatin-A, PF-4, SCYB4

ID gène: 5196

NCBI Accession: NP_002610

UniProt: P02776